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県立広島大学 生物資源科学部(庄原キャンパス)




 Laboratory of Bioscience & Biotechnology for cell Function Control

研究業績Achievements

Original papers
*学会発表等も含めた業績はresearchmapをご覧ください

2020年
1) Saitoh Y*, Umezaki T, Yonekura N, Nakawa A. Resveratrol potentiates intracellular ascorbic acid enrichment through dehydroascorbic acid transport and/or its intracellular reduction in HaCaT cells. Mol Cell Biochem. 2020,467(1-2):57-64. doi: 10.1007/s11010-020-03700-2..*Corresponding author (https://link.springer.com/article/10.1007/s11010-020-03700-2)


2019年
1) Asada R, Saitoh Y, Miwa N. Effects of hydrogen-rich water bath on visceral fat and skin blotch, with boiling-resistant hydrogen bubbles. Med Gas Res. 2019 Apr-Jun;9(2):68-73.


2018年
1) Saitoh Y*, Comments to the article “Artefacts with ascorbate and other redox-active compounds in cell culture: epigenetic modifications, and cell killing due to hydrogen peroxide generation in cell culture media”. Free Radic Res. 2018, 52(9): 910-912. *Corresponding author

2)
Saitoh Y*, Nakawa A, Tanabe T, Akiyama T. The influence of cellular senescence on intracellular vitamin C transport, accumulation, and function. Mol Cell Biochem. 2018, 446(1-2):209-219. doi: 10.1007/s11010-018-3287-y. *Corresponding author

3) Hamada H, Shimoda K, Saitoh Y, Doi S, Fujitaka Y, Ono T, Hamada H, Araki M. Resveratrol oligosaccharides induces mRNA expression for SIRT. Natural product communications 2018, 13(4):455-456.

4)
Tanaka Y, Saitoh Y, Miwa N. Electrolytically generated hydrogen warm water cleanses the keratin-plug-clogged hair-pores and promotes the capillary blood-streams, more markedly than normal warm water does. Medical Gas Research 2018, 8(1):12-18.


2017年
1)Ohwada R, Ozeki Y, Saitoh Y*. High-dose ascorbic acid induces carcinostatic effects through hydrogen peroxide and superoxide anion radical generation-induced cell death and growth arrest in human tongue carcinoma cells. Free Radic Res. 2017 Jul-Aug 51(7-8):684-692. doi: 10.1080/10715762.2017.1361533. (http://www.tandfonline.com/doi/full/10.1080/10715762.2017.1361533)*Corresponding author


2016年
1)Saitoh Y*, Ohta H, Hyoudou S. Protective effects of polyvinylpyrrolidone-wrapped fullerene against intermittent ultraviolet-A irradiation-induced cell injury in HaCaT cells. J Photochem Photobiol B. 2016, 163:22-29. *Corresponding author

2) Li Q, Tanaka Y, Saitoh Y, Miwa N. Effects of Platinum Nanocolloid in Combination with Gamma Irradiation on Normal Human Esophageal Epithelial Cells. J Nanosci Nanotechnol.2016 16:5345-5352.

3) Saitoh Y*, Ikeshima M, Kawasaki N, MasumotoA, Miwa N. Transient generation of hydrogen peroxide is responsible for carcinostatic effects of hydrogen combined with platinum nanocolloid, together with increases intracellular ROS, DNA cleavages, and proportion of G2/M-phase. Free Radical Res. 2016 Apr;50(4):385-95. doi: 10.3109/10715762.2015.1131823.
*Corresponding author


2015年
1) Saitoh Y*, Yoshimoto T, Kato S, Miwa N. Combined treatment with ascorbic acid and a capacitive-resistive electric transfer hyperthermic apparatus induces synergistically carcinostatic effects on Ehrlich ascites tumor cell. Exp Oncol. 2015, 37(2):94-99. *Corresponding author

2) Li Q, Tanaka Y, Saitoh Y, Tanaka H, Miwa N. Carcinostatic effects of platinum nanocolloid combined with gamma irradiation on human esophageal squamous cell carcinoma. Life Sci. 2015, 127:106-114. doi: 10.1016/j.lfs.2015.01.028.

3) Kato S, Kato Y, Shibata H, Saitoh Y, Miwa N. Repressive effects of oat extracts on intracellular lipid-droplet formation in adipocytes and a three-dimensional subcutaneous adipose tissue model. Materials science and engineering C. 2015, C49:269-273.

4) Ishikura H, Ono T, Oki S, Saito Y, Umei N, Tsumiyama W, Tasaka A, Aihara K, Sato Y, Matsumoto T, Otsuka A. Effect of stretch on improvement of muscular contractures in rats. J Phys Ther Sci. 2015, 27(9): 2821–2823. doi: 10.1589/jpts.27.2821


2014年
1) Kato S, Aoshima H, Saitoh Y, Miwa N. Fullerene-C60 derivatives prevent UV-irradiation/TiO2-induced cytotoxicity on keratinocytes and 3D-skin tissues through antioxidant actions. J Nanosci Nanotechnol. 2014, 14(5):3285-3291.

2) Asada R, Liao F, Saitoh Y, Miwa N. Photodynamic anti-cancer effects of fullerene [C60]-PEG complex on fibrosarcomas preferentially over normal fibroblasts in terms of fullerene uptake and cytotoxicity. Mol Cell Biochem. 2014, 390(1-2):175-184.


3)
Haeiwa H, Fujita T, Saitoh Y*, Miwa N.
Oleic acid promotes adaptability against oxidative stress in 3T3-L1 cells through lipohormesis. Mol Cell Biochem. 2014, 386(1-2):73-83. DOI: 10.1007/s11010-013-1846-9. *Corresponding author


2013

1) Saitoh Y, Morishita A, Mito S, Tsujiya T, Miwa N. Senescence-induced increases in intracellular oxidative stress and enhancement of the need for ascorbic acid in human fibroblasts. Mol Cell Biochem. 2013, 380(1-2):129-141. doi: 10.1007/s11010-013-1666-y.


2) Kato S, Saitoh Y, Miwa N. Inhibitions by hydrogen-occluding silica microcluster to melanogenesis in human pigment cells and tyrosinase reaction. J Nanosci Nanotechnol. 2013, 13(1):52-59.

3)  Kato S, Saitoh Y, Miwa N. Repressive effects of a capacitive-resistive electric transfer (CRet) hyperthermic apparatus combined with provitamin C on intracellular lipid-droplets formation in adipocytes. Int J Hyperthermia. 2013, 29(1):30-37.


2012年 
1) Saitoh Y, Mizuno H, Xiao L, Hyoudou S, Kokubo K, Miwa N. Polyhydroxylated fullerene C60(OH) 44 suppresses intracellular lipid accumulation together with repression of intracellular superoxide anion radicals and subsequent PPARγ2 expression during spontaneous differentiation of OP9 preadipocytes into adipocytes. Mol Cell Biochem. 2012, 366(1-2): 191-200.

2) Kato S, Hokama R, Okayasu H, Saitoh Y, Iwai K, Miwa N. Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity. J. Nanosci. Nanotechnol. 2012, 12(5): 4019-4027.

3) Asada R, Kageyama K, Tanaka H, Kimura M, Saitoh Y, Miwa N. Carcinostatic effects of diverse ascorbate derivatives in comparison with aliphatic chain moiety structures: Promotion by combined hyperthermia and reduced cytotoxicity to normal cells. Oncol Lett. 2012, 3(5):1042-1046.

4) Kato S, Saitoh Y, Iwai K, Miwa N.
Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes. J Photochem Photobiol B. 2012, 106(1):24-33.

5) Asada R, Kageyama K, Tanaka H, Saitoh Y, Miwa N. Anti-tumor and anti-invasive effects of diverse musk-fragrant macrocyclic ketones and their enhancement by hyperthermia. Mol Med Report 2012, 5(1):148-152.


2011年

1) Xiao L, Aoshima H, Saitoh Y, Miwa N. Highly hydroxylated fullerene localizes at the cytoskeleton and inhibits oxidative stress in adipocytes and a subcutaneous adipose-tissue equivalent. Free Radic Biol Med. 2011, 51(7):1376-1389.

2) Kato S, Asada R, kageyama K, Saitoh Y, Miwa N. Anticancer effects of 6-O-palmitoyl-ascorbate combined with a capacitive-resistive electric transfer hyperthermic apparatus as compared with ascorbate in relation to ascorbyl radical generation. Cytotechnology 2011, 63(4):425-435.

3) Kato S, Aoshima H, Saitoh Y, Miwa N. Fullerene-C60 incorporated in liposome exerts persistent hydroxyl radical-scavenging activity and cytoprotection in UVA/B-irradiated keratinocytes. J Nanosci Nanotechnol. 2011, 11(5):3814-3823.

4) Liao F, Saitoh Y, Miwa N. Anticancer effects of fullerene [C60] included in polyethylene glycol combined with visible light-irradiation through ROS generation and DNA fragmentation on fibrosarcoma cells with scarce cytotoxicity to normal fibroblasts. Oncol Res. 2011, 19(5):203-216.

5) Kato S, Matsui H, Saitoh Y, Miwa N. Fish collagen-containing drink is subcutaneously absorbed and attenuates the UVA-induced tissue-integrity destruction and DNA damages in 3D-human skin tissue model. J Funct Foods 2011, 3(1):50-55.

6) Saitoh Y, Miyanishi A, Mizuno H, Kato S, Aoshima H, Kokubo K, Miwa N. Super-highly hydroxylated fullerene derivative protects human keratinocytes from UV-induced cell injuries together with the decreases in intracellular ROS generation and DNA damages. J Photochem Photobiol B. 2011, 102(1):69-76.


2010年

1) Asada R, Kageyama K, Tanaka H, Matsui H, Kimura M, Saitoh Y, Miwa N. Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell

death.
Oncol Rep. 2010, 24(6):1463-1470.

2)  Saitoh Y, Xiao L, Mizuno H, Kato S, Aoshima H, Taira H, Kokubo K, Miwa N. Novel polyhydroxylated fullerene suppresses intracellular oxidative stress together with repression of intracellular lipid accumulation during the differentiation of OP9

preadipocytes into adipocytes
. Free Radical Research 2010, 44(9):1072-1081.

3)   Xiao L, Aoshima H, Saitoh Y, Miwa N.  Fullerene-polyvinylpyrrolidone clathrate localizes in the cytoplasm to prevent Ultraviolet-A ray-induced DNA-fragmentation and activation of the transcriptional factor NF-kappa B. J Cell Biochem. 2010,

111(4):955-966.

4)   Kato S, Taira H, Aoshima H, Saitoh Y, Miwa N. Clinical Evaluation of Fullerene-C60 Dissolved in Squalane for Anti-Wrinkle Cosmetics. J Nanosci Nanotechnol. 2010, 10:6769-6774.

5)   Kato S, Aoshima H, Saitoh Y, Miwa N. Defensive effects of fullerene-C60 dissolved in squalane against the 2, 4-nonadienal- induced cell injury in human skin keratinocytes HaCaT and wrinkle formation in 3D-human skin tissue model. J Biomed Nanotechnol. 2010, 6:52-58.

6)    Xiao L, Aoshima H, Saitoh Y, Miwa N. The effect of squalane-dissolved fullerene-C60 on adipogenesis-accompanied

oxidative stress and macrophage activation in a preadipocyte-monocyte co-culture system.
Biomaterials. 2010, 31:5976-5985.

7)    Watanabe S, Saitoh Y, Namba M, Miwa N. Administration with telomeric DNA telomere-like oligonucleotides induces enhancement of telomerase activity and resistance against oxidative stress in telomere reverse transcriptase gene-transfected human fibroblasts. Biomed Pharmacother. 2010, 64(8): 565-571.

8)    SaitohY, Harata Y, Mizuhashi F, Nakajima M, Miwa N. Biological safety of neutral-pH hydrogen-enriched electrolyzed water upon mutagenicity, genotoxicity and subchronic oral toxicity. Toxicol Ind Health 2010, 26(4):203-216.

9)  Kato S, Kikuchi R, Aoshima H, Saitoh Y, Miwa N. Defensive effects of fullerene-C60/liposome complex against UVA-induced intracellular reactive oxygen species generation and cell death in human skin keratinocytes HaCaT, associated with intracellular uptake and extracellular excretion of fullerene-C60. J Photochem Photobiol B. 2010, 98(2):144-151.

10)  Kato S, Aoshima H, Saitoh Y, Miwa N. Fullerene-C60/liposome complex: Defensive effects against UVA-induced damages in skin structure, nucleus and collagen type I/IV fibrils, and the permeability into human skin tissue. J Photochem Photobiol B. 2010, 98(1):99-105.

2009年

1)   Saitoh Y, Yumoto A, Miwa N.alpha-Tocopheryl phosphate suppresses tumor invasion concurrently with dynamic morphological changes and delocalization of cortactin from invadopodia. Int J Oncol. 2009, 35(6):1277-1288. 

2)  Sugimoto M, SaitohY, Ichihashi M, Miwa N. Suppressive effects of phosphorylated ascorbate on ultraviolet B radiation-induced DNA damage and differential expression of wild-type and mutated p53 tumor-suppressor gene in keratinocytes. Mol Med Rep. 2009, 2(6):917-922.

3)   Saitoh Y, Nagai Y, Miwa N.Fucoidan-Vitamin C complex suppresses tumor invasion through the basement membrane, with scarce injuries to normal or tumor cells, via decreases in oxidative stress and matrix metalloproteinases. Int J Oncol. 2009, 35(5):1183-1189.

4)   Aoshima H, SaitohY, Ito S, Yamana S, Miwa N. Safety evaluation of highly purified fullerenes: based on screening of eye, skin and cell damages. J Toxicol Sci. 2009, 34(5):555-562.

5)   Saitoh Y, Yoshimura Y, Nakano K, Miwa N.Platinum nanocolloid-supplemented hydrogen-dissolved water inhibits

growth of human tongue carcinoma cells preferentially over normal cells. Exp Oncol. 2009
, 31(3):156-162.

6)  Kato S, Aoshima H, Saitoh Y, Miwa N. Highly-hydroxylated or γ-cyclodextrin-bicapped water-soluble derivative of fullerene: the antioxidant ability assessed by electron spin resonance method and β-carotene bleaching assay. Bioorg Med Chem Lett. 2009, 19:5293-5296.

7)  Kato S, Aoshima H, Saitoh Y, Miwa N.Biological safety of LipoFullerene composed of squalane and fullerene-C60 upon mutagenesis, photocytotoxicity, and permeability into the human skin tissue. Basic Clin Pharmacol Toxicol. 2009, 104(6):483-487.

8)  Kato S, Aoshima H, Saitoh Y, Miwa N.Biological safety of Liposome Fullerene consisting of hydrogenated lecithin, glycine soja sterols and fullerene-C60 upon photocytotoxicity and bacterial reverse mutagenicity. Toxicol Ind Health. 2009, 25(3):197-203.

9)  Xiao L, Kaneyasu K, Saitoh Y, Terashima Y, Kowata Y, Miwa N.Cytoprotective effects of the lipoidic-liquiform pro-vitamin C tetra-isopalmitoyl-ascorbate (VC-IP) against ultraviolet-A ray-induced injuries in human skin cells together with collagen retention, MMP inhibition and p53 gene repression. J Cell Biochem. 2009, 106(4):589-598.

2008年

1)  Saitoh Y, Okayasu H, Xiao L, Harata Y, Miwa N.Neutral-pH hydrogen-enriched electrolyzed water achieves tumor-preferential clonal-growth-inhibition over normal cells and tumor-invasion inhibition concurrently with intracellular oxidant repression. Oncol Res. 2008, 17(6):247-255.


2007年

1)  Saitoh Y, Masumoto S, Yanada S, Miwa N.Apoptosis prevention in neuronally differentiated PC12 cells, by bcl-2 gene transfection in the non-proliferative and differentiated state, with retention of neuron-specific proteins and blockage of mitocondrion-relayed apoptotic pathway. Gene Ther Mol Biol. 2007, 11:275-288.

2)    Saitoh Y, Fukuoka Y, Nishikimi M, Miwa N. Transfection with glutathione-dependent dehydroascorbate reductase genes exerts cytoprotective effects against hydroperoxide-induced cell injury through vitamin C regeneration and oxidative-stress diminishment. Gene Ther Mol Biol. 2007, 11:143-150.


2006年以前

1)    Yanada S, Misumi M, Saitoh Y, Kaneda Y, Miwa N. Transfection of the anti-apoptotic gene bcl-2 inhibits oxidative stress-induced cell injuries through delaying of NF-κB activation. Gene Ther Mol Biol. 2006, 10:269-276.

2)    Yanada S, Saitoh Y, Kaneda Y, Miwa N. Cytoprotection by bcl-2 gene transfer against ischemic liver injuries together with repressed lipid peroxidation and increased ascorbic acid in livers and serum. J Cell. Biochem. 2004, 93(5):857-870.

3)    Saitoh Y and Miwa N. Cytoprotection of vascular endotheliocytes by phosphorylated ascorbate through suppression of oxidative stress that is generated immediately after post-anoxic reoxygenation or with alkylhydroperoxides. J Cell Biochem. 2004, 93(4):653-663.

4)    Saitoh Y, Ouchida R, Miwa N.Bcl-2 prevents hypoxia / reoxygenation-induced cell death through suppressed generation of reactive oxygen species and upregulation of Bcl-2 proteins. J Cell Biochem. 2003, 90(5):914-924.

5)    Saitoh Y, Ouchida R, Kayasuga A, Miwa N.Anti-apoptotic defense of bcl-2 gene against hydroperoxide-induced cytotoxicity  together  with suppressed lipid peroxidation, enhanced ascorbate uptake, and upregulated Bcl-2 protein. J Cell Biochem. 2003, 89(2):321-334.

6)    Nakamura S, Shibuya M, Saito Y, Nakashima H, Saito F, Higuchi A, Tsubota K.Protective effect of D-beta-hydroxybutyrate on corneal epithelia in dry eye conditions through suppression of apoptosis. Invest Ophthalmol Vis Sci. 2003, 44(11):4682-4688.

7)    Saitoh Y, Nagao N, O'Uchida R, Yamane T, Kageyama K, Muto N, Miwa N.Moderately controlled transport of ascorbate into aortic endothelial cells against slowdown of the cell cycle, decreasing of the concentration or increasing of coexistent glucose as compared with dehydroascorbate. Mol Cell Biochem. 1997, 173(1-2):43-50.

 

Original Papers (in Japanese) 

1) 高濃度アスコルビン酸は過酸化水素およびスーパーオキシドアニオンラジカルによる細胞死誘導と増殖抑制を介してヒト舌がん細胞における抗がん効果を発揮する
大和田 凌平小関 齋藤 靖和 ビタミン 92(5-6), 267-269, 2018

2) ヒト線維芽細胞の老化に伴う細胞内酸化ストレスの増加とアスコルビン酸必要性の亢進
斉藤 靖和*、森下 愛子、水戸 智美、辻屋 翼、三羽 信比古
 ビタミン 88(5-6), 287-288, 2014



総説


1)新規素材の美肌・抗老化・抗メタボ効能に関する化粧品・美肌サプリメントとしての研究開発動向~主に水素・白金・フラーレン・プロビタミンCE・炭酸ガス・天然系抗酸化剤について~ 斉藤 靖和, 加藤 信哉, 肖 黎(Xiao Li, 三羽 信比古Cosmetic Stage 51, 47-62, 2010

2)細胞死抑制遺伝子bcl-2導入による臓器保護; Bcl-2の抗酸化作用を介した細胞死制御 柳田 忍, 斉藤 靖和, 三羽 信比古Organ biology 2009, Vol.16, No.4, 421-426.

3)フラーレン誘導体の化粧品としての効能開発と今後の展開?抗酸化作用と細胞死防御効果をベースとして?Fullerene Derivatives: Exploitation of Cosmetic Effects and Development in the Future ~Based on the Antioxidant Action and Cell-Death Preventive Effects~  (Li Xiao)、河地 信哉、廖 (Feng Liao)、青島 央江、斉藤 靖和、三羽信比古Cosmetic Stage 1巻7号, pp.36-45, 2007

 

Books

1) 黎,鈴木 清香,河地 信哉,松林 賢司,平田 直之,斉藤 靖和,三羽 信比古:遠藤 守信,飯島 澄男 編「ナノカーボンハンドブック」, カーボンナノ素材の皮膚メラニン抑制作用と化粧品への応用.第1編,第3章,3-7-2 ナノカーボン素材の皮膚メラニン抑制作用と化粧品への応用pp.514-5212007株式会社エヌ・ティー・エス ISBN978-4-86043-176-1

2) 峰,甘 学輝,肖 黎,松林 賢司,Robiul Md. Islam斉藤 靖和,三羽信比古:遠藤 守信,飯島 澄男 編「ナノカーボンハンドブック」, 第2編,第3章,3-2-7 フラーレン誘導体の抗酸化作用と細胞死抑制効果および医療応用. pp.660-667, 2007, 株式会社エヌ・ティー・エス ISBN978-4-86043-176-1

3)河村 卓也,斉藤 靖和,加藤 詠子,続木 敏,三羽 信比古:アンチ・エイジングシリーズ2皮膚の抗老化最前線, 3-1-2-2抗しわ活性ビタミンC誘導体と分子メカニズム. pp.201-229, 2006, 株式会社エヌ・ティー・エス ISBN4-86043-104-9

4)斉藤 靖和,柳田 忍,大内田 理佳,三羽 信比古:三羽 信比古・編著 「美肌・皮膚防護とバイオ技術?バイオ化粧品・美肌健康食品・ハイテク美肌機器の最新動向」, 26 アポトーシス抑制遺伝子による細胞死制御?抗アポトーシス遺伝子bcl-2の細胞死抑制メカニズムと皮膚防護のための遺伝子治療への発展. pp. 274-286, 20038, シーエムシー出版 ISBN4-88231-408-8

5)斉藤 靖和,村田 友次,木幡 康則,三羽 信比古:三羽 信比古・編著「バイオ抗酸化剤プロビタミンC 皮膚障害・ガン・老化の防御と実用化研究」,10 抗酸化剤の活性増強?薬剤の組織内浸透性と細胞内取込み亢進. pp. 269-318, 19999, フレグランスジャーナル社 ISBN4-89479-017-3



Others

1) 健康的な肌を目指そう 冬の乾燥子どもは特にケアを 
齋藤 靖和 えんじいな【広島版】2017.12月号 p12. 広島リビング新聞社
 

県立広島大学 生物資源科学部
生命環境学科/総合学術研究科
生命システム科学専攻

〒727-0023
広島県庄原市七塚町5562

TEL 0824-74-1757

E-mail:
ysaito-at-pu-hiroshima.ac.jp
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