【地域資源開発学科】共役リノール酸のリン脂質誘導化とその脂肪蓄積抑制作用

Riska Rian Fauziah, Rie Chin, Shinjiro Ogita, Tomoyuki Yoshino, Yukihiro Yamamoto

The antiadipogenic activity of conjugated linoleic acids (CLA) in the form of phosphatidylcholine-bound (CLA-PC) or free fatty acids (FFA; CLA-FFA) was evaluated using 3T3-L1 adipocytes. Phosphatidylcholine from soya (soy-PC) was used as the comparison of PC form. Both the lipid accumulation and activity of glycerol-3-phosphate dehydrogenase were measured to determine lipogenesis, whereas the glycerol content was measured to evaluate lipolysis. The CLA uptake also measured to find out the utilization of CLA by the cells. As a results, lipid accumulation in 3T3-L1 adipocytes was inhibited in a dose-dependent manner following treatment with CLA-PC (50-400 μM). Both CLA-PC and soy-PC significantly suppressed lipid accumulation compared with CLA-FFA, even though the amount of CLA in CLA-PC was a half than CLA-FFA. The CLA uptake of PC form was superior to FFA form, however, no difference was noted between CLA-PC and soy-PC. These forms exerted their antiadipogenic activity via the suppression of lipogenesis, and not by increasing lipolysis. Short-term treatment, especially in the middle stage of differentiation, was more effective than long-term treatment; especially for CLA-FFA. The antiadipogenic effect of CLA-PC was partially attributed to the chemical structure of the PC molecule. These results provide important information for the utilization of physiologically functional fatty acids and particularly CLA in the food and medical fields.

山本准教授らの研究グループは、抗肥満作用や抗がん作用が知られる共役リノール酸と呼ばれる脂肪酸に注目し、その肥満蓄積防止作用について培養細胞を用いて検証しました。脂肪酸の存在形態として、遊離脂肪酸型やリン脂質型が考えられますが、本研究ではリン脂質型の方が脂肪蓄積に対して有効であることを示唆する結果が得られました。機能性脂肪酸の摂取形態あるいは投与形態を考える上で重要な知見を得たと考えています。